Western Blot:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
Autoradiography:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
RNA Extraction:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
SYBR Green Assay:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
Real-time Polymerase Chain Reaction:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
Sample Prep:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
Chromatin Immunoprecipitation:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
TA Cloning:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
Sequencing:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
RNA Sequencing Assay:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
Cell Culture:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
DNA Methylation Assay:Article Title: Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER NHM_N2 (Melanocytes) HEM-Dark P2 (Passage) Sciencell Cat. # 2220; lot 1446 NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater Promocell Cat. # C-14040; lot 4070201.1 NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater Promocell Cat. # C-14042; lot 5071302.2 451Lu Rockland, Inc. Cat. # 451Lu-01-0001; RRID:CVCL_6357 WM278 Rockland, Inc. Cat. # WM278-01-0001; RRID:CVCL_6473 WM115 Rockland, Inc. Cat. # WM115-01-0001; RRID:CVCL_0040 WM266-4 Rockland, Inc. Cat. # WM266-4-01-0001; RRID:CVCL_2765 WM239A Rockland, Inc. Cat. # WM239A-01-0001; CVCL_6795 WM1552c Rockland, Inc. Cat. # WM1552C-01-0001; RRID:CVCL_6472 WM902b Rockland, Inc. Cat. # WM902B-01-0001; RRID:CVCL_6807 WM983A Rockland, Inc. Cat. # WM983A-01-0001; RRID:CVCL_6808 WM983B Rockland, Inc. Cat. # WM983B-01-0001; RRID:CVCL_6809 WM983C Rockland, Inc. Cat. # WM983C-01-0001; RRID:CVCL_A338 WM1361a Rockland, Inc. Cat. # WM1361A-01-0001; RRID:CVCL_6788 WM793b Dr. Meenhard Herlyn (Wistar) RRID:CVCL_8787 WM35 Dr. Meenhard Herlyn (Wistar) RRID:CVCL_0580 IGR1 Dr. Danny Reinberg (NYU Langone Health) RRID:CVCL_1303 501Mel Dr. Ruth Halaban (Yale University) RRID:CVCL_4633; A375 ATCC Cat. # CRL-1619; RRID:CVCL_0132; SK-MEL-2 ATCC Cat. # HTB-68; RRID:CVCL_0069 SK-MEL-28 ATCC Cat. # HTB-72; RRID:CVCL_0526 SK-MEL-147 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_3876 SK-MEL-173 Dr. Alan Houghton (MSKCC, NY) RRID:CVCL_6090 SK-MEL-239 Dr. Poulikos Poulikakos (MSSM, NY) RRID:CVCL_6122 113/6-4L Dr. Robert Kerbel, (Sunnybrook Health Sciences Center, Toronto) Cruz-Munoz et al., Cancer Research, 2008 HEK293T ATCC Cat. #CRL-3216, RRID:CVCL_0063 Experimental Models: Organisms/Strains Mouse: female, 6 to 8 weeks old, NOD.CgPrkdcscidIl2rgtm1Wjl/SzJ (NSG) Jackson Laboratories 005557; RRID:IMSR_JAX:005557 Oligonucleotides All primer, shRNA, and sgRNA sequences Table S5 N/A hsa-miR-7-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 000386 hsa-miR-671-5p Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 197646_mat RNU6B Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001093 RNU48 Taqman primers Thermo Fisher Scientific Cat. # 4427975; Assay ID: 001006 RNU44 Taqman primers Thermo Fisher Scientific Cat. # 4427975 Assay ID: 001094 hsa-miR-7-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205877 hsa-miR-671-5p LNA PCR primer set, UniRT Exiqon, Inc. Cat. # 205649 cel-miR-39-3p, LNA control primer set, UniRT Exiqon, Inc. Cat. # 203952 (Continued on next page) Cancer Cell 37, 55–70.e1–e15, January 13, 2020 e3
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